Recombinant human basic fibroblast growth factor(bFGF)

★  Product Properties

Biological activity: The biological activity of the mouse fibroblast cell line BALB/3T3 was determined by the method of Chinese Pharmacopoeia 2015 edition using bFGF.

★  Product  Application

It can be used for cell culture to enhance cell proliferation, cell growth and cell differentiation.

★    Usage method

 l      It is recommended to centrifuge the powder immediately before opening the lid to sink the powder to the bottom of the tube, then dissolve it in sterilized deionized water to prepare a storage solution of 100 μg/ml. It can be stored stably for 1 week at 2 °C ~ 8 °C. When diluted to a lower concentration (not less than 10 μg/ml), 0.1% BSA or HSA is required. If it is necessary to extend the storage time, it is recommended to freeze at -70 ° C or -20 ° C. It is not recommended to repeat freezing and thawing.

l   This product is only used for research and should not be used on animals and humans.

★  Transportation and storage Stability

l   Transport stability：It is transported in an ice pack to keep it active.

l   Storage stability：It is stored at -15 ° C to -20 ° C to ensure stability within 24 months.

★  Related products  

 Recombinant human epidermal growth factor (EGF). Recombinant human insulin-like growth factor (IGF).

★  Product  specifications

★ Product Description

  Recombinant human basic fibroblast growth factor (also known as basic FGF, bFGF, FGF2, FGF-β or heparin-binding growth factor) is a biologically active protein suitable for cell culture applications. bFGF regulates various processes such as cell proliferation, differentiation, survival, adhesion, exercise, apoptosis, limb formation and wound healing. bFGF can be used for angiogenesis, fibroblast mitosis, axonal growth of PC-12 cells, receptor binding, and tyrosine phosphorylation studies.

 This product is a lyophilized powder prepared by recombinant E. coli expression, further purified by multiple steps, and then freeze-dried. It can be widely used in the field of cell culture.

★ Product features

l   No animal origin: the product is free of animal virus, no pathogenic substances, no external factor pollution, and high safety.

l      High purity: SDS-PAGE purity reached 98%.

l      High biological activity: 1 x 10^7 IU per 1 mg of protein.

Use a small amount of protein to achieve the desired experimental results.

l   Quality stability: This product is a lyophilized powder containing bioactive protective agent. It has been mass-produced and the difference between batches is small.